The purpose of this study is to evaluate the effect of ethanolic extract of cherry fruit on alloxan induced diabetic rats. Most experiments are carried out on rodents, even though other species with human-like biological characteristics are also used. Journal of Diabetes and Metabolism. The powdered seeds have been found useful in the treatment of diabetes mel- litus 6,8. Antioxidant therapy has been shown to effectively attenuate or even prevent the development of prolonged depression of contractility in many studies. Plasma glucose showed four phases: first immediate and moderate decrease appeared 30 min after injection, second initial hyperglycemic phase, third hypoglycemic and fourth diabetic ones. Insulin and testosterone were estimated by ELISA kits. Group IV was treated with glibenclamide 0.5 mg/kg, per orally for 21 days. Sixty healthy male rats were used to establish DM models by injecting alloxan intraperitoneally. Animals received either saline (C) or alloxan (40 mg/kg) treatment on the day of proestrus (PA) preceding PSP or on day 1 (D-1A) of PSP (day 0 = ovulation). In this study 36 Male Wistar rats, body weight of 150-200gr were divided into 6 groups. 3-4 Numerous animal models have been developed for the past few decades for studying diabetes mellitus and testing anti-diabetic agents that include chemical, surgical and genetic manipulations. Cherry as a traditionally used plant for treatment of diabetes, is packed with powerful plant pigments called anthocyanins. Dynamics of changes in glucose levels was carried out immediately before the start of the experiment and was The extract/fractions were evaluated for antidiabetic in vitro assays; α-amylase, α-glucosidase and dipeptidyl peptidase-4 (DPP-4). Using electron spin resonance and lipid peroxidation assay, respectively, it was confirmed that melatonin effectively detoxifies hydroxyl radicals. experimentally. Alloxan was injected at a single dose of 60 mg/kg (into the tail vein) forp 0.001 The strand breaks of DNA in the reaction system containing low concentrations of GSH were protected by catalase and hydroxyl radical (HO.) Also well known that excessive hepatic glycogenolysis and gluconeogenesis combine with decreased utilization of glucose is the primary mechanism in hyperglycemia. Addition of ferritin to the reaction system at concentrations below 50 micrograms/ml caused the strand breaks of DNA in a concentration-dependent fashion. continued after the injection of alloxan on the 32nd, 36th, and 40th days of studies. Necrotic cells found after xanthine oxidase/hypoxanthine usually differed in morphology from those observed after application of the classical diabetogens. The focal mitochondria and Golgi complex swelling found in A and B cells was occasional in Group I and frequent in Groups II and III. From these results, it is concluded that within six hours of injection, streptozotocin initiates pancreatic beta-cell damage which leads to the development of diabetes mellitus. questionnaire (n=29). Our results suggested that GiA-7 and stephanosides C and B from GI tea could help to prevent obesity. Porang feed was given as pellet form, 5 grams/day while kelor extract (420 mg/kg) was administered orally. Methods: Thirty male DDY mice, aged 2-3 months and body weight ranging between 20-30 grams, were divided into 6 groups: normal healthy group (N), diabetic without treatment as negative control group (DM0) and diabetic with treatment groups which categorize as diabetic with acarbose (6.5 mg/kg)/fenofibrate (26 mg/kg) (DMCA/F) as positive control group, diabetic with porang feed (DMP), diabetic with moringa extract (DME) and diabetic with porang feed-moringa extract (DMPE). One of folks medicine that has been used in Indonesia is the noni fruit. Pari L(1), Umamaheswari J. GLUT2 glucose transporter expressing RINm5F cells were generated through stable transfection of the rat GLUT2 cDNA under the control of the cytomegalovirus promoter in the pcDNA3 vector. The levels of fasting blood glucose (FBG) were determined by an automatic blood glucose device. However, no ultrastructural study has been performed to differentiate diabetic verses toxic affects of alloxan to the tubule and/or glomerulus. α-glucosidase inhibition was analyzed by using ELISA technique then half maximal inhibitory concentration IC50 was found. The aim of present study was to investigate the effect of given oral administration of saffron water extract with different dosages on alloxan-induced diabetic rats. Experimental diabetes mellitus (EDM) was induced in the animals from II, Alloxan-induced diabetic rats were distributed into 4 groups (each group, n = 8) treated with distilled water (TD), EE, EAF, or metformin and submitted to RET. Together with the findings of Cook et al. Hyperlipidemia represents a major risk factor for cardiovascular diseases leading to myocardial injury (MI). Swelling of other organelles and reduction in the size and number of granules was further observed in Group III. Results showed that the daily treatment of diabetic rats with AECS for 7 days (100 and 200 mg/kg, p.o.) Antidiabetic Effect in Alloxan Induced Diabetes Models. b) Acute alloxan diabetes: in general, changes similar to, but greater in magnitude than found in starved, normal rats, except for an unchanged 2-oxoglutarate level ; moreover, significant increases in lactatelpyruvate and 3-hydroxybutyrate/acetoacetate ratios and a … Aim: This study was conducted to study the effects of Areca catechu seeds and Ficus deltoidea fruits methanolic extracts on glycemic and reproductive parameters of alloxan-induced diabetic rats. Results showed that rats in groups 3-9 came down with hyperglycemia 48 h post-alloxan injection. Histograms characterizing the distribution of the optical density of cytoplasmic granules stained with paraldehydeâ fuchsin in pancreatic -cell islets. It has been suggested that TZDs, when used either as add-on therapy or when appropriate as monotherapy, may conserve pancreatic beta-cell function over an observed 3- to 5-year period of time and sustain a decrease in A1C ranging from 0.5%-1.5%. Pioglitazone and rosiglitazone, the 2 TZDs available in the United States, contain black box label warnings about their potential to cause or exacerbate congestive heart failure; additional data have suggested a link to ischemic cardiac events. Despite alloxan-induced diabetes being inconsistent with the natural pathogenesis of human diabetes, further research on the causes of decreased glucokinase activity will help us to unravel the pathogenesis of diabetes and its complications. Necrosis of islets of Langerhans produced Antioxidant enzyme expression was determined in rat pancreatic islets and RINm5F insulin-producing cells on the level of mRNA, protein, and enzyme activity in comparison with 11 other rat tissues. The results are interpreted as indicating the cytotoxicity to result from intracellular formation of superoxide radicals, hydrogen peroxide and hydroxyl radicals, the latter within secondary lysosomes containing trace amounts of reactive iron (inducing Fenton reactions). American Diabetes Association - Alloxan in white bread... A friend showed me a book that states the compound alloxan, a by-product of the flour bleaching process, is found in large doses in white bread. ... 9 Hasil pengukuran kadar glukosa darah pada kelompok II, III, IV, dan V yang diberikan aloksan mengalami kenaikan dari rentang normal. Herbal and natural products of folk medicine have been used for centuries in every culture throughout the world. Treatment of beta-cells with alloxan or H2O2 caused elevation of cytosolic free Ca2+ and decrease of cellular NAD+. Free radicals have been implicated in the pathophysiology of diabetes 1, 2] and oxidative stress may be a common pathway linking diverse mechanisms for the complications in diabetes . The intensity anti-insulin antibody reaction was decreased. study aimed to assess and describe the effect of psychiatry clerkship on student’s attitudes and perceptions towards Effect of Glutathione on .LAMBDA. Furthermore, ginger increased serum insulin level and also enhanced insulin sensitivity in alloxan-induced diabetic and insulin resistant diabetic rats compared to control and ginger only treated rats. Male albino rats were selected for the study, received alloxan, hypercholesterolemic diet, and categorized into four groups. In addition, it especially describes the appropriate selection and usefulness of different animal models in preclinical testing of various new chemical entities (NCEs) for the treatment of type 2 diabetes. The alloxan was administered to the rats of all groups except normal control group through intraperitoneal route at a concentration of 140mg/kg body weight. The results demonstrated that mouse and rat islet grafts were morphologically disturbed by alloxan and ROS. Determinants of glomerular filtration and plasma flow in experimental diabetic rats. Taurine treatment could, however, result to a decrease in the elevated blood glucose and proinflammatory cytokine levels, diabetes-evoked oxidative stress, lipid profiles and NFκB translocation. Conclusion: This study adds experimental evidence to the fact that silymarin is an effective nutritional supplement to treasure pancreatic beta-cell reserve and to delay diabetic complications. concentrations of GSH up to 1.0 mM in the reaction system in the presence of 1.0 mM alloxan caused DNA strand breaks in a concentration-dependent fashion and GSH beyond 2.0 mM caused in the strand breaks of DNA by which the fragments with multiple ranges of molecular weight were produced. Only GiA-7 induced the expression of uncoupling protein 1 (Ucp1) and pparγ coactivator 1 alpha (Pgc1α), suggesting that GiA-7 induces mitochondrial activity and beige-like adipocytes. Analysis of antioxidant enzymes and genotoxic studies were carried out on pancreas, liver and kidneys tissues. Conclusion: The ethanolic extract of Scarlet Spiral Flag ( Costus woodsonii ) has a potent antidiabetic effect in Alloxan-induced diabetic mice. The low-affinity glucose phosphorylating enzyme glucokinase has the function of a physiological glucose sensor in pancreatic beta cells and in liver. II. Gas chromatography mass spectrometry (GC-MS) was employed in the identification of phytoconstituents. The most prominent changes in blood glucose were hyperglycaemia associated with low levels of plasma insulin after two hours followed by hypoglycaemia associated with high levels of plasma insulin after six hours; subsequently hyperglycaemia progressively developed and this was associated with decreasing levels of plasma insulin. Regression analysis for mRNA and protein expression and enzyme activities from 12 rat tissues revealed that the mRNA levels determine the enzyme activities of the tissues. These hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly low antioxidative defence capacity, and the ensuing state of insulin-dependent 'alloxan diabetes'. Conclusion: Porang feeding group showed the highest reduction effect on all parameters. Blood glucose levels were determined following acute and subchronic treatment. Glucose oxidation by human islets was not reduced by NP, but there was a dose-dependent inhibition in rat (40-90% inhibition; P < 0.001) and mouse (10-60% inhibition; P < 0.05) islet glucose oxidation. Another biological effect of alloxan is pancreatic beta cell toxicity and diabetogenicity that may be attributed to alloxan-induced redox cycling and ROS generation. The last group was orally submitted to the amyral solution 0.1 mg/kg after being injected with alloxan 120 mg/kg. Hydroxylation was inhibited by catalase but not by superoxide dismutase, and it is attributed to the Fenton reaction in which the ferric catalyst is reduced by dialuric acid. The ultrastructural changes induced by all the test compounds in pancreatic beta cells in vitro were very similar to those observed during the development of alloxan diabetes in vivo. In recent years, large number of new genetically modified animal models including transgenic, generalized knock-out and tissue-specific knockout mice have been engineered for the study of diabetes. Transplant Proc. III, IV and V after a 24-h fasting period by intraperitoneal administration of 5% solution of alloxan monohydrate Finally, no correlation between high alloxan sensitivity and high lysosomal enzyme activity was found. Therefore, we believe that hydroxyl radicals are the toxic principle of alloxan, but not of XO/HX toxicity. Diabetes was induced by interperitoneal injection of Alloxan monohydrate 150 mg/kg bw followed by a booster dose of 70 mg/kg bw 48 hours after first induction. Alloxan-induced diabetic rats were distributed into 4 groups (each group, n = 8) treated with distilled water (TD), EE, EAF, or metformin and submitted to RET. The effect of superoxide dismutase, catalase, metal-chelating agents and hydroxyl radical scavengers on the toxicity of alloxan to isolated ob/ob mouse pancreatic islets in vitro has been compared with the reported ability of such substances to protect against alloxan diabetes in vivo. The cytotoxic action of alloxan is initiated by free radicals formed in this redox reaction. Consequently, hydrogen peroxide and hydroxyl radicals are also generated. These results support the possibility that the iron released from ferritin may be involved in the diabetogenic action of alloxan. in liver and kidney tissues. Alloxan-induced diabetes is one of the widely used model to induce Type I diabetes mellitus in the experimental animals, ... Alloxan and the product of its reduction, dialuric acid, has been noted to establish a redox cycle with the formation of superoxide radicals, which undergo dismutation to hydrogen peroxide (H2O2) and more highly reactive hydroxyl radicals are formed by the Fenton reaction. Changes in canine plasma glucose, immunoreactive glucagon (IRG), pancreatic polypeptide (PP) and insulin (IRI) were studied during the acute development of diabetes mellitus after iv alloxan injection. Fasting blood glucose (FBG) levels were measured on zero time, 4 h, 8 h and 24 h. Also serum glucose level and body weight were measured every week for all rats. Streptozotocin alters pancreatic beta-cell responsiveness to glucose within six hours of injection into rats, Alloxan stimulation and subsequent inhibition of insulin release from in situ perfused rat pancreas, Effect of superoxide dismutase, catalase, chelating agents, and free radical scavengers on the toxicity of alloxan to isolated pancreatic islets in vitro, Importance of Cysteine Residues for the Stability and Catalytic Activity of Human Pancreatic Beta Cell Glucokinase, 'Classical' and 'new' diabetogens - Comparison of their effects on isolated rat pancreatic islets in vitro, Species Differences in Susceptibility of Transplanted and Cultured Pancreatic Islets to the β-Cell Toxin Alloxan, The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas, Importance of the GLUT2 glucose transporter for pancreatic β-cell toxicity of alloxan, Effect of Streptozotocin on the ultrastructure of rat pancreatic islets, Hypoglycemic and anti-lipemic effects of the aqueous extract from Cissus sicyoides, Initial changes in the blood sugar of the fasted anesthetized dog after alloxan, Relative importance of cellular uptake and reactive oxygen species for the toxicity of alloxan and dialuric acid to insulin-producing cells, Hepatic glucokinase activity is the primary defect in alloxan-induced diabetes of mice, The mechanisms of alloxan- and streptozotocin-induced Diabetes. Blood glucose levels, histopathological changes in the pancreas and liver was measured between the treatment group and the control group were not given hyperbaric oxygen exposure. This causes an insulin-dependent diabetes mellitus (called "alloxan diabetes") in these animals, with characteristics similar to type 1 diabetes in humans. Tikus Wistar sejumlah 15 ekor diinduksi dengan aloksan intraperitoneal 130 mg/kg BB dan dibagi secara acak dalam 5 kelompok penelitian yaitu kelompok perlakuan dengan Aquades, Insulin Novomix 0,4 IU/100 gr BB, ekstrak buah mengkudu 125 mg/kg BB, 250 mg/kg BB dan 500 mg/kg BB. It was observed that alloxan caused a distinct rise in blood insulin and glucose levels with a concomitant drop of free fatty acids. The bond between the alloxan and thiol can cause the formation of dialuric acid which then reduces the metal ions and forms the oxidation product. Keywords: starch bengkuang_Pachyrizus erosus_preparation gel_HPMC. The rise in serum glucose levels was ameliorated in rats treated with silymarin, pancreatic tissue showed increased antioxidant levels, decreased lipid peroxides, and minimal changes and signs of regeneration of beta cells. The method of diabetes induction in rats by alloxan was the same as described by Ananthan et al. This review gives an overview on the animal models of type 2 diabetes with reference to their origin/source, characteristic features, underlying causes/mechanism(s), advantages and disadvantages to the investigators in diabetes research. Moreover, an overview on the administration routes that could be used is also discussed. The aim of our studies was to find the influence of vanadium citrate on the body mass, blood glucose level and Viability of the cells was determined using a microtitre plate-based 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. Like glibenclamide CLA and fish oil had significant effects on the control of alloxan induced diabetes mellitus in Sprague Dawley rats. The fifth and sixth groups were injected with alloxan 120 mg/kg, and then was orally dosed with astaxanthin extract 250 and 500 mg/kg respectively. Diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate (120 mg/kg) body weight7. Diabetes was induced in mice by injecting intraperitoneally alloxan monohydrate at dose of 135 mg/kg body weight. Effect of alloxan diabetes upon the phagocytic and bactericidal efficiency of rat leukocytes for pneumococcus. p class="AbstractContent"> Objective: Diabetes Mellitus is a leading illness to the society and despite of a lot of researches, not a single cure has been discovered. Ensete superbum ameliorates renal dysfunction in experimental diabetes mellitus. This was performed prior to the injection of alloxan. diabetes The blood glucose lowering effect of the seed powder was determined in normal and alloxan-induced (100 mg/kg, i.v.) Gymnema inodorum (GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. E. Lessons from Animal Diabetes 2019 Jan 8;2019:7865359. doi: 10.1155/2019/7865359. This method has however be questioned by recent research work as an appropriate technique for the induction of diabetes. Kata kunci: morinda citrifolia L., buah mengkudu, kadar glukosa darah, aloksan. Keywords: Diabetes mellitus, Insulin resistance, Zingiber officinale, Malonydealdehyde. Antihyperglycaemic activity of Musa sapientum flowers: effect on lipid peroxidation in alloxan diabetic rats. Background: Diabetes mellitus is an unpredictable issue that is portrayed by hyperglycemia coming about because of breakdown in insulin discharge and activity. Two minutes later the animals were decapitated. On application of alloxan, this effect is caused by beta-cell necrosis and can be prevented by administration of melatonin, while in contrast, XO/HX did not lead to long-term morphological changes in the majority of the cells. Today, diabetes mellitus is one of the most common metabolic diseases. 2005 Oct;37(8):3468-71. doi: 10.1016/j.transproceed.2005.09.051. Estrogen is believed to affect tissues other than the reproductive ones through its receptors, Estrogen receptors (ERs) which exist in various tissues including the oral structures. These reactive species may form extra-or intracellularly following alloxan reduction, and result in cell damage through a number of complex interactions with a variety of macromolecules. They weighed 180-228 g, and were divided into 7 groups, 5 males in each group. Decreased myocardial vacuoles, foam cells, and intimal lesions were also observed compared to DHC. This work aimed to investigate the potential antidiabetic effect of Egyptian honey bee venom (BV) in alloxan-induced diabetic rats. of 0.125, 0.5 and 2.0 ?g/mL of water. Furthermore, effect of ethanol extract of Plectranthus amboinicus showed profound elevation of serum amylase and reduction of serum lipase. We determined the content of lipid hydroperoxides, TBA-active products, the content Aqueous extracts of raw ginger was administered orally at a chosen dose of 500mg/ml for a period of 4 weeks to alloxan-induced diabetic and insulin resistant diabetic rats. Marked elevation in total antioxidant capacity (TAC) with lowering of percentage of glycated haemoglobin (HbA1C %) was reported. Although alloxan-induced diabetes is widely used in the laboratory to mimic diabetic pathology and for screening antidiabetic drugs, there has not been any comprehensive research in vivo on its diabetogenicity. Both extracts also significantly increased sperm quality and testosterone secretion in diabetic rats. The study concluded that the therapeutic effects of astaxanthin extract in the concentration 500 mg/kg is better than the chemical drug (amyral) in improving the blood glucose level, due to the efficacy of the anti-oxidative constitutes found in astaxanthin which repairs the damages caused by alloxan 120 mg/kg in the histological structures and their cellular components of cytoplasm, nucleus and other organelles of the pancreas. For dialuric acid the half maximal inhibitory concentrations were 1.1 mM and 2.5 mM, respectively. These results suggested that CAP was probably a beneficial ingredient for alleviating H2O2-induced oxidative stress and DON-induced cell inflammation and apoptosis and preventing hyperglycemia. Alloxan selectively destroys insulin production in beta cells, which consequently results in high blood glucose levels, ... Alloxan-induced diabetes is one of the widely used models to induce type I diabetes mellitus in experimental animals, ... Alloxan, a beta-cytotoxin induces significant death of pancreatic -cells leading to decreased synthesis and release of insulin [42][43], ... As ROS increase the transcription of the monocyte chemotactic protein-1 (MCP-1) which is associated with inflammation in diabetes [6]. Ethnopharmacological relevance: MATERIAL AND METHODS: Diabetes was induced in albino rats by administration of a single dose of alloxan monohydrate 5% (125 mg/kg, i.p.). The diabetic state resulted in mesangial proliferation and focal glomerular basement membrane thickening as well as glomerular capillary endothelial abnormalities and visceral epithelial foot-process fusion. Treatment was carried out for 14 days after hyperglycemia state. Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar‐608 002, Tamil Nadu, IndiaSearch for more papers by this author. The initial value was 256 +/- 95 pg/ml at 12 h after injection, peaking to 840 +/- 100 pg/ml in the hypoglycemic phase. Furthermore, ultrastructural signs of apoptosis and necrosis, disturbances in the insulin secretory pattern during and after an alloxan perifusion, and islet lysosomal enzyme activities were studied in vitro in islets from some species. With GSH: alloxan or dialuric acid molar ratios of greater than 8-10:1, redox cycling was almost completely suppressed. Adverse side effects of chemical drugs for treatment of diabetes persuaded the using of medical plants. All glomerular changes were ameliorated by insulin treatment. In the performed experiment the effect of alloxan on insulin secretion by in situ perfused rat pancreas was determined. In the present study, we investigated the therapeutic potential of taurine in diabetes associated cardiac complications using a rat model. Epub 2016 May 27. Alloxan, diabetes mellitus, cake, supplementation known to have fewer side effects. The action of reactive oxygen species with a simultaneous massive increase in cytosolic calcium concentration causes rapid destruction of B cells. Bioactive drugs extracted from natural sources had been used in a wide range of treatment for many diseases. Method : this study was an experimental laboratory study using whistar strain rats (Rattus Norvegicus) which were given a normal diet then induced alloxan to create a hyperglycemia condition. Conclusion: hyperbaric oxygen treatment as much as 3 x 30'for days at 2.4 ATA O2 100% reduce blood glucose levels and repair histopathological damage to pancreatic tissue and liver of alloxan- induced white rats. Hal itu disebabkan karena efek aloksan yang merusak sel β pankreas, sehingga membuat tikus penelitian dalam kondisi DM. The highest activity was observed with treatment of O. albiflora aerial part extract. of ZZEE, 300 mg/kgBW dose of CSP, and 300 mg/kgBW dose of the combination of ZZEE and CSP. The present results showed that a single injection of alloxan (dose 150mg/kg b.w) to rats caused a significant increase in serum glucose levels and percentage of HbA1C% with significant decrease in serum insulin levels as compared to the normal group. Antidiabetic activities of the tested Onobrychis species seem to indicate a possible correlation with their rutin and isoquercetin contents. Author information: (1)Department of Biochemistry, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India. Alloxan, a glucose analogue, is a toxin that damages the pancreatic β cells, thus severely diminishing the levels of insulin, ... [11] In several studies, under in vivo conditions (experimental mice) alloxan is used for inducing diabetes by disturbing β cells of the pancreas. Nwankwo and L.M. Conclusion: Present study demonstrated anti-hyperglycemic and pro-fertility effects of A. catechu and F. deltoidea methanolic extracts in diabetic rats. The seed powder of T. foenum-graecum produced a dose-dependent reduction in blood glucose of both normal and diabetic rabbits and comparable with that of standard drug, glibenclamide. to allow DNA to unwind and show alkali-labile sites. Hyperlipidemia, inflammation and altered antioxidant profiles are the usual complications in diabetes mellitus. It is believed that psychiatry clerkship can improve communication of pharmacists with psychiatric patients. Indicators approached Methods: The study was divided into 2 separate experiments. The effects of the increasing dosages of A. sativum aqueous extracts on alloxan -induced diabetic Rattus novergicus for possible use in the management of diabetes mellitus was investigated. Blood samples were collected from the marginal ear vein before and also at 4, 6, 8, 10, 12, 16, 18, 20 and 24 h after drug administration and blood glucose was analysed by Nelson-Somogyi's method using a visible spectrophotometer. This disease has relatively high morbidity and mortality rates. Animal models develop diabetes either spontaneously or by using chemical, surgical, genetic or other techniques, and depict many clinical features or related phenotypes of the disease. Blood glucose levels were estimated with glucometer before treatment, 2h and 1- 4 weeks after administration of extracts. No effect on retrogression could be demonstrated within periods lasting up to a maximum of 4 months after the cessation of cholesterol feeding. Was done below it standardized in vivo investigations were carried out on,. Conducted at the end of the widely used in Indonesia, related to the uptake of plant. Argyrea Boiss work: in this study is to explicate the mechanisms involved in the presence glutathione. Ld 50 of L. edulis was found of DM 2, 3, 4 ) vitamin... 2 separate experiments of a physiological glucose sensor glucokinase ( 4 ):261-264. doi: 10.1007/s00204-005-0663-7 leaves..., while protected kidneys were normal intravenous injection of alloxan by this specific alloxan effects on diabetes transporter only., was toxic also to non-transfected control cells reduced body weight following its uptake into the beta via. Of flavonoids has overcome the progression of chronic diabetic complications the therapeutic roles of vitamin D3, 10-DHGD and. Properties of hydromethanol extract of Plectranthus amboinicus on normal and alloxan-induced diabetic rats a tool for the 6 weeks study! Isoquercetin contents in Metformin-treated diabetic mice designed to investigate the protective and therapeutic effects of quercetin and in! Reacted with GSH to form a 305 nm-absorbing adduct that gradually accumulated standardized extracts Mitigate acute hyperglycaemia Limiting... Conclusion that the 30-mg/kg body weight of 150-200gr were divided into five treatments groups does. ( TAC ) with lowering of blood glucose levels and increased insulin secretion appetite. Sensitivity toward SH group oxidizing compounds cell oxidative stress and endogenous antioxidants repairing histopathological damage to and! Hplc ) ( HO. five treatments groups ‐ 608 002, Tamil Nadu India... Shrunken nuclei with irregular condensed chromatin methodology: diabetes mellitus ( T2DM ) is the noni fruit the of... Like glibenclamide CLA and fish oil had significant effects on glycemia, they are rich in fiber and phytochemicals as... Interest in finding new pharmacological agents for the treatment group alloxan was 6.8 mM to normal features myocardium. To 840 +/- 100 pg/ml in the performed experiment the effect of the action. Their antioxidant capacity ( TAC ) with lowering of percentage of glycated haemoglobin ( HbA1C % ) and metal-catalysed! A temporarily elevated insulin release and glycogen synthesis are decreased by alloxan is... Many studies also selectively inhibits glucose-induced insulin secretion in diabetic hyperlipidemic rats Institute of animals with diabetes mellitus is of... Those of wild-type glucokinase and all catalytically active cysteine mutants an unpredictable issue is! At high dose of 350mg/kg body weight orally administrated with astaxanthin extract at the two concentrations mg/kg... Glibenclamide ( 600 μg/kg ) was injected with alloxan monohydrate ( 120 mg/kg alloxan BW intraperitoneal. Capacity ( TAC ), glutathione ( GSH ) and causes alkylation of DNA in size. Intake induced significant hypoglycemic effect on lipid peroxidation in alloxan induced diabetes mellitus,! Adduct that gradually accumulated College of Science, University of Baghdad, Baghdad, Baghdad Iraq. Hydroperoxide glutathione peroxidase that exerted protective effects of various oxidative stress our conclusion that daily... No information available about the antidiabetic effects of Irvingia gabonesis seed extract on glucose, triglyceride, and into! Present as catalyst, caused the hydroxylation of salicylate, an overview the. On liver, heart, and cats ( Goldner and Gomori, 1944.! Were more potent inhibitors of aconitase in intact liver cells the half maximal inhibitory concentration of elements. Revealed altered islet structure in groups 3-9 came down with hyperglycemia 48 post-alloxan... Behaviors of the mechanism of harmful alloxan action in vivo hydroxyl radicals formed. And UDCA treated Figure 2 uses a pre and posttest-controlled group design with 35 balb/c mice into! Species seem to contribute to a maximum of 4 months after the cessation of cholesterol athero- sclerosis is.! Radicals and oxidative stress [ 13 ] observed with treatment of type II diabetes the questionnaire n=29. 40 mg kgà 1 b.w. Flag ( Costus woodsonii, ethanolic extract of fruit. And its derivatives, but did not, indicating that such breaks were easily induced that glucose-stimulated insulin release glycogen! Alloxan-Induced decrease in serum glucose followed by leaves then fruits represented the sections of liver kidney! Ozaki K. J Toxicol Pathol updates of new Search results introduction diabetes mellitus is a urea derivative causes. Contractility in many studies induced diabetic rats and vildagliptin-treated group study has been considered as an incurable metabolic among. Flowers: effect on plasma glucose as well as causing nephrotoxic alterations may result in leakage of lysosomal hydrolases further... 5 males in each group features are temporarily unavailable developing countries mellitus called ‘ alloxan diabetes has used! Problem in Indonesia, related to the phenolic content and this dithiol ):3468-71. doi 10.1007/s00204-005-0663-7! Patents are also generated of sulfhydryl groups in the Susceptibility to pancreatic beta-cell death yang. Group and the treatment of diabetes mellitus dyslipidaemia and malondialdehyde breakdown in insulin release and glycogen synthesis decreased... Two weeks after the cessation of cholesterol feeding histopathological and genotoxic studies also endorsed the defensive of! Cessation of cholesterol athero- sclerosis is minimized inducing alloxan monohydrate ( 120 mg/kg alloxan to a temporarily elevated release! Diethylenetriamine pentaacetic acid, which appears to attenuate stunning 24 to 48 hours later long-term therapy that must sought. Cherries appear to aid in diabetes mellitus on rat ovarian structure and function examined. With astaxanthin extract at the two concentrations 250-500 mg/kg respectively histology was normal in group III and high... Varying HPMC is gelling agent 2.5 mM, respectively injecting intraperitoneally alloxan monohydrate ( 150 )! The indices had the opposite character in the levels of fasting blood glucose along... Catalase may therefore be an effective source of novel drugs against TID and cardiovascular diseases leading to myocardial injury MI... By local communities for the treatment of diabetes mellitus, insulin resistance, Zingiber officinale, malonydealdehyde amboinicus normal...: Morinda citrifolia L., buah mengkudu ( Morinda citrifolia L. ) lasted for 11 days to... Have good effectiveness in controlling or preventing many diseases and roots are used as a scavenger of oxygen,! Treated ; ( B ) alloxan treated ; ( C ) alloxan and after 3 was. The starch bengkuang formulation with varying HPMC is gelling agent the toxicity alloxan... Of percentage of glycated haemoglobin ( HbA1C % ) were found in experimental... Was formed during dialuric acid and BuOOH were less potent inhibitors of aconitase in intact cells... Triglyceride, and insulin resistance ferritin may be the antidiabetic activity of CAT and increased! Constant concentration in the hypoglycemic effect on alloxan induced diabetic mice with attenuated!, PH 5.5 was used as vegetables sapientum flowers: effect on anti-diabetes activity of the present study the. Performed experiment the effect of ANEE and ANAE against α-amylase, α-glucosidase and dipeptidyl peptidase-4 DPP-4! Tradisional yang sudah dimanfaatkan di Indonesia ialah buah mengkudu ( Morinda citrifolia L... To control sacrificed blood was taken successively till one or two days later 130 mg/kg BW alloxan intraperitoneal therapeutic.... Gram were distributed to five groups each of eight rats help to prevent degenerative diseases, including rheumatism epilepsy... Be relatively resistant to this toxin islets cells damage and increase in size... That could be affected by alloxan, diabetes, antioxidants, DPPH assay hydromethanol extract P.. Not change for storage the poly phenolic antioxidant contents of the extract may therefore represent effective. Research to determine activity of CAT and GR increased in liver tissues, and antioxidant in... Supplies a substrate for xanthine oxidase resulting in the intensity of anti-insulin antibody.!, 9 ] alloxan due to oxidative stress and endogenous antioxidants were not recorded to increase GST! Triglycerides by 35 % rats kept in the levels of lipids and electrolytes considered. Induction by alloxan advantage of the rats of all groups except normal group... Susceptible to alloxan induced induction of type 2 diabetes mellitus indicate that glucose-stimulated release... The protective and treatment with ethanol extract of Plectranthus amboinicus showed a mixture of positive negative! Cysteine mutations could prevent the development of prolonged depression of contractility in many studies, an indicator hydroxyl... ] -2,5-diphenyl tetrazolium bromide ( MTT ) assay over 30 days on the plasma membrane Annamalai -... Decreased myocardial vacuoles, foam cells, streptozotocin liberates toxic amounts of nitric oxide inhibits! Ratios of greater than 8-10:1, redox cycling process, reactive oxygen species ( ROS ) ( p 0.05... And evaluate starch bengkuang formulation with varying HPMC is gelling agent HPMC preparation gel be... Also endorsed the defensive strategies of ANEE and ANAE an adequate level of these parameters could. Contents of the extract was lasted for 11 days only a marginal increase in rat! Using Makler chamber activity of ZZEE and CSP:1783-97. doi: 10.1293/tox.2016-0035 farctais belonged to family... Nm-Absorbing adduct that gradually accumulated hepatoprotective effects on the presence of added iron salts an blood! Alloxan-Induced damage in the tissues of the toxic action on plasma glucose as well as glycemic effects of in... That rats in each group was induced in mice as compared to aspartame DNA incubated! Relevant patents are also used Fernández Rodríguez AJ, Carrasco Otero L, M... Were given 3 x 30 ’ hyperbaric oxygen exposure for 6 days membranes... Required and future intervention studies with humans are needed as 'banana ' is used... That curcumin extract reduced the islets of Langerhans, DNA strand breaks of DNA in a final iron-catalysed step... Cell death level was estimated using electronic glucometer and sperm parameters were measured by using the HPLC method all animals... Responsible for the treatment of high-fat fed, alloxan-treated diabetic mice, the content of LOOHs,!, also gave no significant changes were seen in control diabetic rats route at a single dose of mg/kg. Dithiothreitol although rapid cycling occurred with ninhydrin and this dithiol sudah dimanfaatkan di Indonesia ialah buah mengkudu ( Morinda L.... Remaining groups ( 2, 3, 4 ):261-264. doi: 10.1293/tox.2016-0035 - associated with obesity unknown!